Abstract
Objective: In the present research work silver nanoparticles were synthesized using the flower extract of Chrysanthemum coronarium and their in-vitro antibacterial activity was evaluated against both the gram-positive S. aureus and gram-negative bacteria E. coli. The flower extract acted both as a reducing as well as a capping agent.Methods: Silver nanoparticles were verified using various spectroanalytical techniques such as visible ultraviolet spectroscopy, zeta potential, fourier transform infrared spectroscopy and particle size analyser. The antibacterial activity was evaluated against both the gram-positive bacteria S. aureus and gram-negative bacteria E. coli using the agar well diffusion method.Results: The silver nanoparticles synthesized were confirmed by the visual colour change. The ultraviolet, visible spectroscopy showed a surface plasmon resonance at 430 nm. Zetapotential was found to be around-15.6mV where the negative value indicated that the synthesized silver nanoparticles are stable. Fourier transform infrared spectroscopy showed the functional groups responsible for the stabilization of the nanoparticles. Particle size analyser showed that the size of the nanoparticles ranged from 5-50 nm. The antibacterial activity of the silver nanoparticles which was performed against S. aureus and E. coli showed good inhibition against both the bacteria. Better antibacterial activity was found for E. coli in comparison to S. aureus as the zone of inhibition for E. coli was found to be at 12 mm at 50ug/ml whereas the zone of inhibition against S. aureus was found to be at 10 mm.Conclusion: The silver nanoparticles were successfully synthesized using a green approach and can be used as a potential resource for therapeutic purpose.
Highlights
Nanoparticles exhibit completely new or improved properties which is based on the specific characteristics such as size, shape and orientation [1]
Silver nanoparticles were synthesized using the flower extract of Chrysanthemum coronarium which belongs to the family of Asteraceae
Similar results were reported in a research where the silver nanoparticles were synthesized using pomegranate juice extract and it was observed that the gram-negative bacteria E. coli showed a larger zone of inhibition ie 14 mm in comparison to the grampositive bacteria S. aureus, which showed a zone of inhibition of 12 mm at 50ug/ml, this may be due to the variation in the cell wall composition [17]
Summary
Nanoparticles exhibit completely new or improved properties which is based on the specific characteristics such as size, shape and orientation [1]. Nanoparticles attract more researchers for future developments in the area of medicine, healthcare and agriculture due to their diverse, remarkable and captivating range of biological applications [5]. These procedures are eco-friendly and costeffective and has gained a lot of attention owing to the instinctive features such as the usage of natural resources, rapidness, ecofriendliness and benignancy. Chrysanthemum coronarium is a plant of Asteraceae family which contains various bioactive compounds that are used for the treatment of various diseases It has medicinal properties as the leaves are expectorant and stomachic while the flowers are stomachic [6]
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