Bio-analytical Estimation of Metformin Co-administered with Antidiabetic and Antihyperlipidemic Herbal Preparations by LCMS

Abstract

The antidiabetic and antihyperlipidemic herbal preparations were developed in liquid and oral tablet dosage form as Saptarangi Plus Kadha (SK) and Saptarangi Tablet (ST). Streptozotocin (STZ) (45mg/kg) was injected by single intraperitoneal (i.p.) injection and diabetes was induced in Wistar rats of group II to group VII. Metformin (100mg/kg, p.o.) was co-administered orally to group IV to VII with SK and ST as a reference standard drug. There is a need of bio-analytical study and standardization of herbal preparation which is required to get approval of the regulatory agencies for the commercialization. The estimation of metformin was carried in the presence of other herbal phytoconstituents by LCMS/MS. The samples of all the groups were centrifuged and vortexed. The samples were prepared in ACN: 0.1% formic acid (70:30) and LCMS method was developed and validated. The metformin was estimated in presence of active phytoconstituents of SK and ST. SK and ST contains Salacinol and folia salaciosides E1 which was detected by LCMS at m/z 333.42 and m/z 504.63 respectively. There has been an honest effort to create and validate LCMS method for the detection of metformin in presence of phytoconstituents of SK and ST in serum.