Binding of leachable components of polymethyl methacrylate (PMMA) and peptide on modified SPR chip

M Szaloki,Cs Hegedus,G Vitalyos,J Harfalvi

doi:10.1088/1757-899x/47/1/012002

Abstract

Many types of polymers are often used in dentistry, which may cause allergic reaction, mainly methyl methacrylate allergy due to the leachable, degradable components of polymerized dental products. The aim of this study was to investigate the interaction between the leachable components of PMMA and peptides by Fourier-transform Surface Plasmon Resonance (FT SPR). In our previous work binding of oligopeptides (Ph.D.-7 and Ph.D.-12 Peptide Library Kit) was investigated to PMMA surface by phage display technique. It was found that oligopeptides bounded specifically to PMMA surface. The most common amino acids were leucine and proline inside the amino acids sequences of DNA of phages. The binding of haptens, as formaldehyde and methacrylic acid, to frequent amino acids was to investigate on the modified gold SPR chip. Self assembled monolayer (SAM) modified the surface of gold chip and ensured the specific binding between the haptens and amino acids. It was found that amino acids bounded to modified SPR gold and the haptens bounded to amino acids by creating multilayer on the chip surface. By the application of phage display and SPR modern bioanalytical methods the interaction between allergens and peptides can be investigated.

Highlights

Polymers have a major role in most areas of dentistry
Preparation of polymethyl methacrylate (PMMA) The Scanning Electron Microscope (SEM) pictures were taken from the polymer beads and surface fracture of polymerized PMMA (Figure 1.)
Investigation of amino-1-hexanethiol hydrochloride (AHT)-Leu-methacrylic acid (MA) bindings The modified SPR chip (AHT modified) was inserted into the SPR instruments, and the surface was washed by PBS buffer (Figure 3.)

Summary

Introduction

Polymers have a major role in most areas of dentistry. Their properties allow a range of clinical applications not possible with other types of materials. The most common applications of polymers in dentistry include impression materials, aesthetic restorative materials, denture teeth, cements, dies, provisional crowns, endodontic fillings, tissue conditioners, pit, and fissure sealants. The polymethyl methacrylate (PMMA) denture base material is cured from methyl methacrylate (MMA) monomer by a free radical polymerization. This polymerization can be activated either by heating or chemically or both. The conversion of MMA monomers to PMMA polymer is not complete and some free monomer content remains in the polymer.

Objectives

Methods

Results

Conclusion