BINDING OF IMMUNOGLOBULIN-BEARING LYMPHOID CELLS TO ALLOGENEIC AND SYNGENEIC TARGET CELLS

Abstract

This chapter discusses the binding of immune and normal mouse spleen or peritoneal exudate cells (PEL) to a variety of tumor cells. In a study described in the chapter, BALB/c mice were immunized against C57BL/6 leukemia EL4, and the number of spleen cells conjugating with EL4 and with A/J leukemia YAC was determined following centrifugation of mixtures containing spleen and tumor cells, 250 g 10 min at room temperature. The number of cells capable of specifically binding to EL4 increased with time, peaking around day 10. A low but significant level of conjugation was observed with YAC or when nonimmunized spleen cells were examined. To characterize the cell types involved in conjugation, normal and immune spleen cells were stained with fluorescent-labeled antiserum to mouse immunoglobulin before or after conjugating with EL4 or YAC cells. About 29% of immune and 54% of normal conjugated spleen cells were Ig-positive. When the GAMG-F1 treated spleen cells displayed their surface Ig in caps, the caps localized opposite the junctional regions.