Abstract
Sugar sensing and continuous monitoring of glucose (CGM) play an important and vital role in controlling diabetes. Even though present enzyme-based sugar sensors are doing well, research is going on globally for the design of new sensors to address some of their limitations. Among many, sensors based on fluorescent intensity change are drawing more attention; especially those which are based on boronic acid derivatives are more popular because of their ability to reversibly bind diol-containing compounds. Here we have investigated the binding ability of two boronic acid derivatives namely, 5-chloro-2-methoxyphenylboronic acid [5CMPBA] and 2-methoxypyridin-3-yl-3-boronic acid [2MPBA] with mono and disaccharides (sugars) in aqueous medium at physiological pH7.4. The sugar concentration is kept nearly 1000 times more than that of boronic acid. Binding constants (Ka) and dissociation constants (KD) are calculated using Benesi-Hildebrand equation and Lineweaver-Burk equation. The effect of intramolecular hydrogen bonding on fluorescence intensity is observed. The higher value of Ka in case of monosugars indicates the role of structural change in sugar that arises due to mutarotation in binding interactions.
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