Abstract
Rabbit liver microsomal preparations fortified with 0.1 mM NADPH effectively promote hydroxylation of [3β- 3H]- or [24- 14]allochenodeoxycholic acid or [5α.6α- 3H 2]5α-cholestane-3α,7α-diol to their respective 12α-hydroxyl derivatives in yields of about 25 or 65% in 60 min. Minor amounts of other products are formed from the diol. The requirements for activity of rabbit liver microsomal 12α-hydroxylase resemble those of rat liver microsomes. Of a number of enzyme inhibitors studied only p-chloromercuribenzoate demonstrated a marked ability to inhibit the reaction with either tritiated substrate. There was no difference in the quantity of product produced from the tritiated acid or the 14C-labeled acid. No clear sex difference was found in activity of the enzyme, nor was an appreciable difference noted in activity of the enzyme between mature and immature animals.
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