Abstract
A strip-type glucose biosensor, prepared using screen-printing technology and comprising glucose oxidase (E.C.1.1.3.4.), peroxidase (E.C.1.1.3.13.) and ferrocyanide as mediator incorporated into graphite-hydroxyethyl cellulose matrices is described. The sensor acted at 0·0 V vs Ag/AgCl electrode, and the response time was 50–60 s. The calibration was linear up to 25 m m of glucose. The sensor response was constant in the range of pH 7·0–8·5. At 25°C the biosensor temperature coefficient was 2·7% °C −1. The sensor was insensitive to a physiological level of ascorbic acid (40 μ m and was used for glucose determination in whole blood.
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