Abstract
Background: Our study was applied aiming to detect the biofilm formation by the Enteropathogenic E. coli causing diarrhea and to investigate the relation between the Bfp adhesions and EspA filament containing strains with the severity of diarrhea in children under 5 years in Mansoura University Children Hospital, Egypt. Methods: It was carried out by identification of EPEC, Serological agglutination test, Biofilm Formation under static condition at 3,6,9,24 hours. Results: Out of 103 children with diarrhea, 50 E. coli were isolated, 16 (32%) were EPEC and 34 (68%) were non-EPEC. The CFU mean± SD of biofilm of 16 EPEC isolates at 4 time points 3, 6, 9, 24h are 108- 1013, 69.5910±24.9310, 106-109, 20.2948±3.96778, 107-1011, 13.92×1010±3.376×1010, 109-1015, 6.402×1013± 2.496×1013. The relation of the biofilm formation and the severity of diarrhea in 16 EPEC isolates including 5 typical EPEC isolates and11 atypical EPEC reveals significant relation between the duration of the diarrhea and biofilm formation P=0.001**. Conclusion: EPEC is capable of forming biofilms on plastic under static and flow through-continuous conditions which correlate to the severity of the diarrhea in children under 5 years.
Highlights
EPEC was first known as a cause of infantile diarrhea in the 1940s’
EPEC is capable of forming biofilms on plastic under static and flow through-continuous conditions which correlate to the severity of the diarrhea in children under 5 years
There was a decline in typical EPEC prevalence paralleled by an increase in atypical EPEC infections In the twenty-first century [3]
Summary
EPEC was first known as a cause of infantile diarrhea in the 1940s’. It was associated with outbreaks in hospitals and nurseries. Mortality rates of EPEC 25– 50% were reported in the United States and the United Kingdom. EPEC has been categorized into two subgroups termed typical (tEPEC) and atypical (aEPEC). There was a decline in typical EPEC (tEPEC) prevalence paralleled by an increase in atypical EPEC (aEPEC) infections In the twenty-first century [3]. Conclusion: EPEC is capable of forming biofilms on plastic under static and flow through-continuous conditions which correlate to the severity of the diarrhea in children under 5 years
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