Beta-amyloidogenic peptides protect against Herpes Simplex Virus-1 (HSV-1) infections

Abstract

Event Abstract Back to Event Beta-amyloidogenic peptides protect against Herpes Simplex Virus-1 (HSV-1) infections Karine Bourgade-Navarro1*, Geneviève Giroux2, Christian Bocti3, Aurélie Le Page1, Gilles Dupuis4, Eric H. Frost2 and Tamàs Fülöp1 1 University of Sherbrooke, Research Center on Aging, Canada 2 University of Sherbrooke, Microbiology and Infectious Diseases, Canada 3 University of Sherbrooke, Division of Neurology, Canada 4 University of Sherbrooke, Biochemistry, Canada The causes of Alzheimer’s disease (AD) are debated. Experimental data and epidemiological studies suggest that Herpes Simplex Virus-1 (HSV-1) infection is a risk factor. HSV-1 stays latent in trigeminal neurons and can be reactivated under low immune status of the host. AD is characterized by deposits of neurofibrillar tangles and senile plaques which lead to cortical neurodegenerescence. Senile plaques found extracellularly in brains of patients contain the fibrillar form of beta-amyloidogenic Aβ1-40 and Aβ1-42 peptides generated by presenelin cleavage of amyloid precursor protein (APP). Interestingly, herpetic encephalitis caused by Herpes viruses affects the same brain regions as AD. The question is open whether beta-amyloidogenic peptides also possess a physiological role. In this connection, they display anti-bacterial and anti-yeast activities in vitro. We cultured fibroblast (MRC5) and epithelial (A549) cell lines in the presence of amyloidogenic peptides Aβ1-40 and Aβ1-42 added before, simultaneously or after challenge with HSV-1. DNA was extracted and viral replication quantified by real-time PCR 24 h later. Controls were scrambled peptides and antimicrobial peptide LL-37. Results showed that peptides Aβ1-40 and Aβ1-42 inhibited HSV-1 replication, depending on their sequence of addition. For instance, they showed efficiency when added 2 h before or simultaneously to virus challenge. However, they were inefficient when added 2 h or 6 h after HSV-1. In contrast, LL-37 inhibited viral replication under all conditions. Scrambled peptides had no activity. Our data suggest that amyloidogenic peptides Aβ1-40 and Aβ1-42 may belong to a novel class of antimicrobial peptides (AMP) that possess antiviral activity. Keywords: Beta-amyloidogenic peptides, Herpes Simplex Virus, anti-viral replication, antimicrobial peptides, Alzheimer’s disease Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Bourgade-Navarro K, Giroux G, Bocti C, Le Page A, Dupuis G, Frost EH and Fülöp T (2013). Beta-amyloidogenic peptides protect against Herpes Simplex Virus-1 (HSV-1) infections. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00259 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 13 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Mrs. Karine Bourgade-Navarro, University of Sherbrooke, Research Center on Aging, Sherbrooke, Québec, J1H 5N4, Canada, karine.n.bourgade@usherbrooke.ca Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Karine Bourgade-Navarro Geneviève Giroux Christian Bocti Aurélie Le Page Gilles Dupuis Eric H Frost Tamàs Fülöp Google Karine Bourgade-Navarro Geneviève Giroux Christian Bocti Aurélie Le Page Gilles Dupuis Eric H Frost Tamàs Fülöp Google Scholar Karine Bourgade-Navarro Geneviève Giroux Christian Bocti Aurélie Le Page Gilles Dupuis Eric H Frost Tamàs Fülöp PubMed Karine Bourgade-Navarro Geneviève Giroux Christian Bocti Aurélie Le Page Gilles Dupuis Eric H Frost Tamàs Fülöp Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.