Abstract
Biomimetic microspheres containing alginate/carboxymethylcellulose/gelatin and coated with 0%, 1%, 3%, and 6% berberine (BACG, BACG-1B, BACG-3B, BACG-6B) were prepared by the oil-in-water emulsion method combined with spray drying. Through a series of physicochemical parameters and determination of hemostatic properties in vitro and in vivo, the results indicated that BACG and BACG-Bs were effective in inducing platelet adhesion/aggregation and promoting the hemostatic potential due to their biomimetic structure and rough surface. In addition, BACG-6B with high berberine proportion presented better hemostatic performance compared with the commercial hemostatic agent compound microporous polysaccharide hemostatic powder (CMPHP). BACG-6B also showed strong antibacterial activity in the in vitro test. The hemolysis test and cytotoxicity evaluation further revealed that the novel composite biomaterials have good hemocompatibility and biocompatibility. Thus, BACG-6B provides a new strategy for developing a due-functional (hemostat/antibacterial) biomedical material, which may have broad and promising applications in the future.
Highlights
Accepted: 18 January 2021Traumatic massive bleeding has been the leading cause of death in surgery and war [1,2,3,4,5,6]
Biomimetic composite microspheres were prepared using alginate, carboxymethylBiomimeticcellulose, composite microspheres were prepared usingwith alginate, carboxymethyland gelatin as raw materials coated berberine
Hemostasis analysis in vitro and in vivo further proved that biomimetic alginate/carboxymethylcellulose/gelatin (BACG)-6B could both significantly shorten the bleeding time and reduce the amount of bleeding
Summary
Traumatic massive bleeding has been the leading cause of death in surgery and war [1,2,3,4,5,6]. We found a series of SCC-B microspheres containing berberine that simultaneously facilitate hemostatic and antibacterial performance [33] These results confirmed our speculation and further encouraged us to develop novel composite microspheres coated with berberine. SA has strong swelling capacity, SCC can activate erythrocytes and platelets, and gelatin can support a stable and biocompatible skeleton, promoting platelet aggregation [34,35] Combining these three materials helps in sharing their advantages and avoiding their disadvantages. On this basis, berberine was further added to prepare novel due-functional (antibacterial/hemostatic) biomaterials. The composite structure of biomimetic microspheres, their physical properties (such as swelling and degradation), and their functional studies (including hemostatic performance in vitro/in vivo, interaction of platelets, antibacterial activity, and biocompatibility) are comprehensively investigated. Schematic overview overview of of BACG-B scaffolds and its possible cross-linking mechanism
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