Abstract
BackgroundBerberine is known to improve glucose and lipid metabolism disorders, but it poorly absorbed into the blood stream from the gut. Therefore, the exact underlying mechanism for berberine is still unknown. In this study, we investigated the effect of berberine on glucose metabolism in diabetic rats and tested the hypothesis that berberine acts directly in the terminal ileums.MethodsRats were divided into a control group, diabetic group (DM), low dose of berberine group (BerL) and high dose of berberine group (BerH). Ileum samples were analyzed using a Roche NimbleGen mRNA array, qPCR and immunohistochemistry.ResultsWe found that 8 weeks of treatment with berberine significantly decreased fasting blood glucose levels. An oral glucose tolerance test (OGTT) showed that blood glucose was significantly reduced in the BerL and BerH groups before and at 30 min, 60 min and 120 min after oral glucose administration. Plasma postprandial glucagon-like peptide-1 (GLP-1) levels were increased in the berberine-treated groups. The ileum from the BerH group had 2112 genes with significantly changed expression (780 increased, 1332 decreased). KEGG pathway analyses indicated that all differentially expressed genes included 9 KEGG pathways. The top two pathways were the MAPK signaling pathway and the GnRH signaling pathway. Q-RT-PCR and immunohistochemistry verified that glucagon-like peptide 1 receptor (Glp1r) and mitogen activated protein kinase 10 (Mapk10) were significantly up-regulated, in contrast, gonadotropin releasing hormone receptor (Gnrhr) and gonadotropin-releasing hormone 1 (Gnrh1) were down-regulated in the BerH group.ConclusionOur data suggest that berberine can improve blood glucose levels in diabetic rats. The mechanisms involved may be in the MAPK and GnRh-Glp-1 pathways in the ileum.
Highlights
Berberine is known to improve glucose and lipid metabolism disorders, but it poorly absorbed into the blood stream from the gut
The area under the curve (AUC) of oral glucose tolerance test (OGTT) for the DM, BerL and BerH groups increased compared to the control group (P < 0.05)
In this study, we found that the administration of berberine to diabetic rats significantly reduced fasting blood glucose, moderated glucose tolerance and reduced serum insulin
Summary
Berberine is known to improve glucose and lipid metabolism disorders, but it poorly absorbed into the blood stream from the gut. We investigated the effect of berberine on glucose metabolism in diabetic rats and tested the hypothesis that berberine acts directly in the terminal ileums. Diabetes is a disorder of the metabolism of carbohydrates, lipids and proteins in which the body cannot produce insulin or cannot use it to its full potential. Diabetes therapy is centered upon the control of Rhuzima Coptidis was recorded as an anti-diabetes medication approximately 1500 years ago in a book titled “Note of Elite Physicians” by Hongjing Tao. Berberine is the major active component of Rhizoma coptidis, and many studies have been published on the glucose reducing mechanisms of berberine.
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