Abstract
Once pulp necrosis or apical periodontitis occurs on immature teeth, the weak root and open root apex are challenging to clinicians. Berberine (BBR) is a potential medicine for bone disorders, therefore, we proposed to apply BBR in root canals to enhance root repair in immature teeth. An in vivo model of immature teeth with apical periodontitis was established in rats, and root canals were filled with BBR, calcium hydroxide or sterilized saline for 3 weeks. The shape of the roots was analyzed by micro-computed tomography and histological staining. In vitro, BBR was introduced into stem cells from apical papilla (SCAPs). Osteogenic differentiation of stem cells from apical papilla was investigated by alkaline phosphatase activity, mineralization ability, and gene expression of osteogenic makers. The signaling pathway, which regulated the osteogenesis of SCAPs was evaluated by quantitative real time PCR, Western blot analysis, and immunofluorescence. In rats treated with BBR, more tissue was formed, with longer roots, thicker root walls, and smaller apex diameters. In addition, we found that BBR promoted SCAPs osteogenesis in a time-dependent and concentration-dependent manner. BBR induced the expression of β-catenin and enhanced β-catenin entering into the nucleus, to up-regulate more runt-related nuclear factor 2 downstream. BBR enhanced root repair in immature teeth with apical periodontitis by activating the canonical Wnt/β-catenin pathway in SCAPs.
Highlights
During the early years, young patient’s teeth are in development.Pulp necrosis and apical periodontitis (AP) as a consequence of trauma or caries arrest root development in injured immature permanent teeth.[1]
BBR promotes hSCAPs osteogenesis in a time-dependent and concentration-dependent manner To further explore the mechanism of BBR enhancing tissue regeneration in vivo, hSCAPs were separated from the apical papilla of immature third molars (Fig. 3a)
The current study showed that BBR enhanced root repair in immature teeth with AP
Summary
Yujia Cui[1], Jing Xie 2, Yujie Fu1, Chuwen Li3, Liwei Zheng[4], Dingming Huang[1], Changchun Zhou[5], Jianxun Sun[1] and Xuedong Zhou[1]. Once pulp necrosis or apical periodontitis occurs on immature teeth, the weak root and open root apex are challenging to clinicians. An in vivo model of immature teeth with apical periodontitis was established in rats, and root canals were filled with BBR, calcium hydroxide or sterilized saline for 3 weeks. BBR enhanced root repair in immature teeth with apical periodontitis by activating the canonical Wnt/β-catenin pathway in SCAPs. 1234567890();,: INTRODUCTION During the early years, young patient’s teeth are in development.
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