Über den polysaccharidgehalt der serumeiweiss-fraktionen bei gesunden und kranken

Abstract

1. 1. By means of protein- and carbohydrate-staining following paper electrophoretic fractionation of serum from healthy and sick persons, as well as by the determination of the protein-linked hexoses and mucoproteins of these sera, both the relative % content of protein and carbohydrate in the single fractions and the polysaccharide content of 100 g protein from each fraction, have been ascertained In addition, the mucoprotein portion in the α 1-globulin fraction and the quotient of neutral: acid carbohydrate of this fraction have been determined. 2. 2. The albumin-, α 1- and β-globulin fractions contain usually an average of about 20%, the α 2-globulin fraction about 27% and the γ-globulin fraction about 12% of the protein-linked polysaccharides. The standard deviation is greater than with the protein fractions. The albumin protein contains about 1 g%, the α 1-globulin protein about 12 g% carbohydrates, of which 31% belong to the carbohydrates of mucoproteins. The polysaccharide content of the α 2-globulin protein amounts to about 11 g%, that of the β-globulin protein to about 5 g%, and in 100 g γ-globulin protein there are about 2, g carbohydrates. 3. 3. Appropriate average values are given for patients with epidemic hepatitis, chronic hepatitis, cirrhosis of the liver, cholecystitis, carcinoma of various parts, Diabetes mellitus, and disseminated Hodgkin's disease, together with single values for cases with tuberculosis of the lungs, β-plamocytoma and lupus, and also examination results obtained at intervals on patients with cirrhosis of the liver with and without complications, and in one case of β-plasmocytoma. Carcinoma patients have been sub-divided into 3 groups on the basis of these results in conformity with the clinical condition. The diagnostic, differential diagnostic and prognostic importance is touched on. 4. 4. The liver is important as the site of formation of the mucoproteins, which are possibly necessary for the building up of the cell. Mucoproteins have been found in cerebrospinal fluid, in ascites, in the liquid obtained by thoracic empyema puncture, and in leucocytes. No statement can be made about the site of formation and function of the protein-bound hexoses. In two cases of impaired blood supply less proteinbound hexoses were found in arterial blood than in venous blood. 5. 5. The carbohydrate content of the neutral glycoproteids in the α 1-globulin fraction is greater in liver diseases and ulcus duodeni or ventriculi, and lower in other diseases investigated. 6. 6. The particular significance of the carbohydrate content of the γ-globulin fraction is briefly discussed.