Abstract
Photodynamic therapy (PDT) is a promising cancer treatment modality that uses dye-sensitized photooxidation of biologic matter in target tissue. This study explored effects of the photosensitizer BCPD-17 during PDT for osteosarcoma. LM-8 osteosarcoma cells were treated with BCPD-17 and cell viability after laser irradiation was assessed in vitro with the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. The effects of BCPD-17 during PDT recurrence were then examined on tumor-bearing mice in vivo. BCPD-17 had dose- dependent cytotoxic effects on LM-8 osteosarcoma cells after laser irradiation which also had energy-dependent effects on the cells. The rate of local recurrence was reduced when marginal resection of mice tumors was followed by BCPD-17-mediated PDT. Our results indicated BCPD-17-mediated PDT in combination with marginal resection of tumors is a potentially new effective treatment for osteosarcoma.
Highlights
Photodynamic light therapy (PDT) is a two-stage procedure, where a photosensitizer is injected into localized cancer lesions and the tumor is irradiated (Moser, 1998; Ackroyd et al, 2001; Agostinis et al, 2011)
The cells were incubated for 24 hours, and their viability was assessed by the 3-(4, 5-dimethylthiazol2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, which was quantitated based on optical density (OD) with the Bio-Rad Smart Spec 3000 multiwall scanning spectrophotometer (Hercules, CA, USA)
Cells treated with 2 μg/ml BCPD-17 and 6 J/cm2 irradiation appeared similar to the control group
Summary
Photodynamic light therapy (PDT) is a two-stage procedure, where a photosensitizer is injected into localized cancer lesions and the tumor is irradiated (Moser, 1998; Ackroyd et al, 2001; Agostinis et al, 2011). This treatment is highly localized and is not affected by radioresistance or chemoresistance. There has been no previous report about effect of BCPD-17-mediated PDT to suppress local recurrence after tumor resection on osteosarcomas. We hypothesized that photosensitizer BCPD-17-mediated PDT could be a promising treatment to suppress local recurrence after tumor resection for osteosarcoma. In an in vivo study we tested the ability of BCPD-17-mediated PDT to suppress local recurrence after tumor resection
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