Abstract
BackgroundMatrix-assisted laser desorption/ionization (MALDI) is a soft ionization mass spectrometric technique, allowing the analysis of bio-molecules and other macromolecules. The matrix molecules require certain characteristic features to serve in the laser desorption/ionization mechanism. Therefore, only a limited number of compounds have been identified as ultraviolet- laser desorption/ionization (UV-LDI) matrices. However, many of these routine matrices generate background signals that useful information is often lost in them. We have reported flavones, coumarindione and benzimidazole derivatives as alternate UV-LDI matrices.ResultsThirty one compounds have been successfully employed by us as matrices for the analysis of low molecular weight (LMW) peptides (up to 2000 Da). Two peptides, bradykinin and renin substrate tetra-decapeptide were analyzed by using the newly developed matrices. The MS measurements were made after mixing the matrix solution with analyte by using dried droplet sample preparation procedures. The synthesized matrix materials showed better S/N ratios and minimal background signals for low mass range. Furthermore, pico molar concentrations of [Glu1]-fibrinopeptide B human could be easily analyzed with these matrices. Finally, BSA-digest was analyzed and identified through database search against Swiss-Prot by using Mascot.ConclusionsThese results validate the good performance of the synthesized UV-laser desorption/ionization (LDI) matrices for the analysis of low molecular weight peptides.
Highlights
Matrix-assisted laser desorption/ionization (MALDI)-MS was developed in the late 1980s and since it has become a powerful tool for the analysis of large biomolecules, such as proteins, peptides, and nucleic acids, etc
In an attempt to correlate the performance of a matrix in a MALDI experiment with its chemical structure, we studied thirty one synthetic alternate matrices for their desorption and ionization of intact small peptides
Wavelength maxima of synthetic matrix materials were measured to verify their ability to absorb the laser energy at proper λmax employed in ultraviolet-Matrix -assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS)
Summary
Thirty one compounds have been successfully employed by us as matrices for the analysis of low molecular weight (LMW) peptides (up to 2000 Da). Bradykinin and renin substrate tetra-decapeptide were analyzed by using the newly developed matrices. The MS measurements were made after mixing the matrix solution with analyte by using dried droplet sample preparation procedures. The synthesized matrix materials showed better S/N ratios and minimal background signals for low mass range. Pico molar concentrations of [Glu1]-fibrinopeptide B human could be analyzed with these matrices. BSA-digest was analyzed and identified through database search against Swiss-Prot by using Mascot
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