Abstract
Matrix-assisted laser desorption/ionization (MALDI) is a preferred and widely used mass spectrometric technique for the analysis of macromolecules. Limited UV-LDI matrices are available for the analysis of biomolecules due to the restricted structural features to serve in the laser desorption/ionization mechanism with a problem of background signals appearing in the low mass region. This paper describes the application of Schiff base derivatives of acylhydrazide and isatin as alternate UV-LDI matrices for the analysis of peptides with significantly low background signals. Thirty one compounds have been successfully employed as matrices for the analysis of low molecular weight (LMW) peptides (α-Cyano-4-hydroxycinnamic acid (HCCA), a preferred choice for peptide analysis.
Highlights
Soft ionization by matrix-assisted laser desorption/ionization mass spectrometry (MALDI) has become very important in mass spectrometric analysis, for biological macromolecules [1]
Limited UV-LDI matrices are available for the analysis of biomolecules due to the restricted structural features to serve in the laser desorption/ionization mechanism with a problem of background signals appearing in the low mass region
This paper describes the application of Schiff base derivatives of acylhydrazide and isatin as alternate UV-LDI matrices for the analysis of peptides with significantly low background signals
Summary
Soft ionization by matrix-assisted laser desorption/ionization mass spectrometry (MALDI) has become very important in mass spectrometric analysis, for biological macromolecules [1]. Compounds having functional groups other than carboxylic acid have the tendency to be employed as MALDI matrices, for example, 3-aminoquinoline is a good matrix for polysaccharides and proteins [7], 2,4,6-trihydroxy acetophenone [8] and the laser dye coumarin 120 [9] has been found useful in MALDI-MS of oligonucleotides and monosulfated oligosaccharides, respectively. Martic et al [12] have prepared allylated derivatives of DHB, HCCA and caffeic acid for the efficient analysis of compounds by MALDI-MS in low molecular weight region. We have explored new classes of Schiff bases as alternate UV-LDI matrices for the analysis of low molecular weight peptides by MALDI-TOF-MS and to correlate the performance of a matrix with its chemical structure. A large number of Schiff base derivatives of acylhydrazide and isatin with necessary functionalities have been screened as potential matrices
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