Beneficial effects of ε-polylysine on growth performance, antioxidant capacity, intestinal morphology, and gut microbiota in broilers fed a lysine-deficient diet.

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This study was conducted to investigate the effects of dietary ε-polylysine supplementation on growth performance, antioxidant capacity, intestinal morphology, and gut microbiota in broilers fed a lysine-deficient diet. A total of 960 one-day-old yellow-feathered broilers were randomly allocated into six groups with eight replicates and 20 birds per replicate. Birds in the six groups were fed a lysine-deficient basal diet (-0.20 % lysine, NC) or basal diets supplemented with 0.20 % L-lysine hydrochloride (normal lysine level, PC) or varying levels (0.10 %, 0.20 %, 0.30 %, or 0.40 %) of ε-polylysine hydrochloride for 10 weeks. Results showed that compared with the NC group, dietary supplementation with 0.10 % and 0.20 % of ε-polylysine improved growth performance, meat quality and intestinal morphology, evidenced by increased average daily gain, decreased feed-to-gain ratio, increased water holding capacity of breast muscle, and increased jejunal and ileal villus height in ε-polylysine-treated broilers (P < 0.05). In addition, 0.10 % and 0.20 % of ε-polylysine supplementation improved serum nitrogen metabolism, immune and antioxidant capacity to varying degrees. No significant diffrences in growth performance, meat quality, or intestinal morphology were observed among PC and ε-polylysine-supplemented groups (P > 0.05). Interestingly, compared with the PC group, dietary 0.10 % of ε-polylysine positively altered the microbiota composition of broilers, evidenced by the increased the relative abundance of beneficial bacteria (e.g., Faecalibacterium and Phascolarctobacterium) and decreased the abundance of harmful bacteria (e.g., Lachnoclostridium and Peptococcus). In conclusion, diets supplemented with ε-polylysine have beneficial effects on growth performance, meat quality, and gut health of broilers via replenishing lysine or favorably altering immune and antioxidant capacity and gut microbiota. The recommended supplemental amount of ε-polylysine in lysine-deficient diets (L-lysine content in the starter and growing diet is 0.95 % and 0.80 % of the basal diet, respectively) is 0.10 %-0.20 %.