Abstract
Two novel Gram-staining-negative, aerobic, cocci-shaped, non-motile, non-spore forming, pink-pigmented bacteria designated strains T6T and T18T, were isolated from a biocrust (biological soil crust) sample from the vicinity of the Tabernas Desert (Spain). Both strains were catalase-positive and oxidase-negative, and grew under mesophilic, neutrophilic and non-halophilic conditions. According to the 16S rRNA gene sequences, strains T6T and T18T showed similarities with Belnapia rosea CGMCC 1.10758T and Belnapia moabensis CP2CT (98.11 and 98.55% gene sequence similarity, respectively). The DNA G+C content was 69.80 and 68.96% for strains T6T and T18T, respectively; the average nucleotide identity by blast (ANIb) and digital DNA–DNA hybridization (dDDH) values confirmed their adscription to two novel species within the genus Belnapia. The predominant fatty acids were summed feature 8 (C18 : 1ω7c/C18 : 1ω6c), C16 : 0, C18 : 1 2-OH and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c). According to he results of the polyphasic study, strains T6T and T18T represent two novel species in the genus Belnapia (which currently includes only three species), for which names Belnapia mucosa sp. nov. (type strain T6T = CECT 30228T=DSM 112073T) and Belnapia arida sp. nov. (type strain T18T=CECT 30229T=DSM 112074T) are proposed, respectively.
Highlights
Two novel Gram-s taining-negative, aerobic, cocci-s haped, non-motile, non-s pore forming, pink-p igmented bacteria designated strains T6T and T18T, were isolated from a biocrust sample from the vicinity of the Tabernas Desert (Spain)
The genus Belnapia was first described by Reddy et al [1] and it is, at the time of writing, comprised of three species, which were all isolated from soil samples: Belnapia moabensis [1], Belnapia rosea [2] and Belnapia soli [3]
In this study we describe the polyphasic characterization of two strains, namely T6T and T18T, which were isolated from biocrust samples from south-eastern Spain during a study on the microbial diversity of European arid regions
Summary
Two novel Gram-s taining-negative, aerobic, cocci-s haped, non-motile, non-s pore forming, pink-p igmented bacteria designated strains T6T and T18T, were isolated from a biocrust (biological soil crust) sample from the vicinity of the Tabernas Desert (Spain). On the basis of the results from phylogenetic, phenotypic and chemotaxonomic analysis, it is concluded that strains T6T and T18T are related to members of the genus Belnapia and representatives of two novel species.
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