Behavioural responses to pressure changes in cultured Atlantic cod ( Gadus morhua): Defining practical limits for submerging and lifting sea-cages

Abstract

Farmed Atlantic cod ( Gadus morhua) are occasionally exposed to buoyancy changes in sea-cages, through lifting or lowering of cage nets. Physiological processes regulate the level of gas in the closed swim bladders of cod and thus the ability of cod to control their buoyancy. Rapid net lifting may cause positive buoyancy, leading to barotrauma, while net lowering may lead to negative buoyancy and alter cod behaviours. We tested how groups of farmed cod responded immediately after lifting events from 5 different start depths equivalent to 40% pressure reductions, and how long they took to return to pre-lifting pressure levels. In addition, we tested immediate responses and recovery times to cage lowering events equivalent to 100–300% pressure increases. Trials were conducted with 100 cod of 1.1–1.7 kg in a 63 m 3 sea-cage at the lower (5 °C) and upper (16 °C) water temperature limits experienced during culture. Swimming behaviours were measured at fixed intervals before and after cage lifting or lowering, and a feeding test was used to assess appetite. In general, lifting events increased swimming speeds 1.5–4 times and tail beats 2–3 times and fish swam with an average −14° head-down angle, indicating positive buoyancy. The depth before lifting affected the immediate response as the fish became more active after lifting events from shallow compared to deeper depths. Appetite levels decreased for about 2 h after cage lifting, independent of temperature or start depth. The overall recovery time of 8 h after lifting did not depend upon start depth or temperature. Lowering events appeared to cause negative buoyancy. Swimming speeds (1.3–2.3 times) and tail beat frequencies (1.4–2.3 times) increased immediately after cage lowering, and cod swam with an average 30° head-up swimming angle. Neither pressure level nor temperature affected this immediate response. Time to recover to neutral buoyancy for 300% pressure increases took 42–90 h, but only 18–34 h for 100% pressure increases. We conclude that a 40% pressure reduction is an upper limit for lifts of healthy farmed cod. Secondary lifts should not be done until at least 10 h after the first lift. Cage lowering should be done slowly to avoid potentially stressful crowding of negatively buoyant fish on the cage bottom, especially at low temperatures.