Abstract
Observations upon the growth of four strains of rubella virus in human diploid cell strains (HDCS) are reported. Sixtythree cell strains, derived from 29 fetuses by means of an organ culture technique, were studied. All HDCS tested were susceptible to rubella virus, and a chronic infection could be established readily in them. The virus multiplied and was continuously produced at a low level for a maximum of 27 weeks while cell subcultivation proceeded normally. Stationary cell populations produced virus for at least 30 weeks. Experiments to define the growth curves of rubella virus were performed in skin and lung cell strains. Strains of lung cell origin yielded greater amounts of virus than skin cell strains. Both growth curve and chronic infection experiments showed that a maximum of approximately 30% of the cells were infected at any given time. Pharyngeal mucosa cells were stored for three months in the frozen state while chronically infected with rubella. Their life history and virus yield after resuscitation did not differ from unfrozen cells.
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