Abstract
A human diploid fibroblast-like cell strain, TIG-1, which has normal female karyotype, was established and characterized. The average in vitro lifespan is 67 population doublings. Changes in surface area, contents of protein and DNA, doubling time, saturation density, the efficiency of cell attachment and the ability of cell proliferation in a colony were examined in the course of cellular aging in culture. Characteristics of G6PD and LDH were also determined. Hydrocortisone prolongs the lifespan of this cell strain when the hormone is added to the medium in physiological concentrations.
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