Behavior of the P1.HTR mastocytoma cell line implanted in the chorioallantoic membrane of chick embryos

S.F Avram,M Raica,A.M Cimpean

doi:10.1590/s0100-879x2013005002434

Abstract

The P1.HTR cell line includes highly transfectable cells derived from P815 mastocytoma cells originating from mouse breast tissue. Despite its widespread use in immunogenic studies, no data are available about the behavior of P1.HTR cells in the chick embryo chorioallantoic membrane model. The objective of the present investigation was to study the effects of P1.HTR cells implanted on the chorioallantoic membrane of chick embryos. We inoculated P1.HTR cells into the previously prepared chick embryo chorioallantoic membrane and observed the early and late effects of these cells by stereomicroscopy, histochemistry and immunohistochemistry. A highly angiotropic and angiogenic effect occurred early after inoculation and a tumorigenic potential with the development of mastocytoma keeping well mast cells immunophenotype was detected later during the development. The P1.HTR mastocytoma cell line is a good tool for the development of the chick embryo chorioallantoic membrane mastocytoma model and also for other studies concerning the involvement of blood vessels. The chick embryo chorioallantoic membrane model of mastocytoma retains the mast cell immunophenotype under experimental conditions and could be used as an experimental tool for in vivo preliminary testing of antitumor and antivascular drugs.

Highlights

Mast cells are normal residents of tissues [1] and have been recognized as key cells of type I hypersensitivity reactions by expressing critical effector functions in classic IgE-associated allergic disorders [2]
Despite its widespread use in immunogenic studies, no data are available about the behavior of P1.HTR cells in the chick embryo chorioallantoic membrane model
The P1.HTR mastocytoma cell line is a good tool for the development of the chick embryo chorioallantoic membrane mastocytoma model and for other studies concerning the involvement of blood vessels

Summary

Introduction

Mast cells are normal residents of tissues [1] and have been recognized as key cells of type I hypersensitivity reactions by expressing critical effector functions in classic IgE-associated allergic disorders [2]. Mast cells exert distinct non-immunological functions, being involved in tissue homeostasis, fibrosis and angiogenesis. The involvement of mast cells in angiogenesis has been strongly supported by clinico-pathological [3,4,5] and experimental studies [6,7] in different malignant and non-malignant diseases, but their role in the angiogenic process is still not well characterized. The involvement of mast cells in angiogenesis is supported by the ability of these cells to secrete growth factors and molecules that induce vascular hyperpermeability [8]. Between 1976 and 2011, up to 15 published articles have reported the different effects of mast cells or some of their components on the chick embryo chorioallantoic membrane model regarding angiogenesis and inflammation but no chorioallantoic membrane mastocytoma model was established

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