Abstract
Hemolytic behavior of human erythrocytes in water-amide solutions was investigated. Complete hemolysis of erythrocytes occurred in all formamide, DMF, DMA, DEA, and DEF solutions. Sodium chloride was effective in preventing complete hemolysis in solutions containing up to 10% formamide, 20% DMF, 20% DMA, 8% DEA, and 0.2% DEF. The addition of sodium chloride to solutions containing more than the above concentrations of amide did not prevent hemolysis, discoloration, and/or precipitation of human red blood cells. The addition of isotonic phosphate buffer (pH 7) to solutions increased the critical concentration at which the amide solutions damaged red cells. When possible, the data were used to calculate van't Hoff i values for sodium chloride in the various amide solutions.
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