Abstract
Hemolytic behavior of human erythrocytes in watertetramethylurea (TMU) solutions was investigated. TMU freely permeates human red blood cells; however, hemolysis can be prevented by the inclusion of 0.9% sodium chloride in solutions containing up to 8% TMU. The addition of sodium chloride to solutions containing more than 8% TMU did not prevent hemolysis, discoloration, and precipitation of blood components. Divalent and trivalent anions gave greater protection against hemolysis than did the sodium chloride. When possible, the data were used to calculate van't Hoff i values for sodium chloride in aqueous solutions.
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