Abstract
Hemolytic behavior of human erythrocytes in water-DMSO solutions was investigated. Complete hemolysis of erythrocytes took place in all DMSO solutions, with discoloration and precipitation occurring at DMSO concentrations greater than 35 per cent. The addition of 0.9 per cent NaCl or isotonic concentrations of other compounds (CaCl2, dextrose, lactose, KBr, Na citrate, NaBr, Nal, and Na salicylate) prevented hemolysis in solutions containing 0.0 to 40 per cent DMSO. The addition of these compounds to solutions containing more than 40 per cent DMSO did not prevent hemolysis, discoloration, or precipitation of human red blood cells. When possible, the data were used to calculate van't Hoff i values for sodium chloride in various aqueous DMSO solutions. The DMSO concentration which afforded the greatest protection to erythrocytes against osmotic hemolysis was 26–27 per cent. In aqueous ternary solvent systems, DMSO did not affect the concentrations at which propylene glycol and polyethylene glycol 300 damage erythrocytes.
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