Behavior of 14C-diethylene glycol in rat and guinea pig

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14C-Labeled diethylene glycol was synthesized from radioactive ethylene glycol with a good yield and specific radioactivity.As a result of animal experiments using this labeled compound, the following findings were made:1) After single oral administration, the peak of radioactivity in blood was found at 1h, and, thereafter, the radioactivity decreased slowly. The radioactivity in tissues and organs persisted for a long period. A high radioactive concentration was detected in stomach and just relatively high concentrations were observed in liver, pancreas and kidney. About 55% of the dose was excreted in urine by 24h after administration.2) The radioactivity in tissues distributed evenly after repeated oral administration, as in the case of single administration, and accumulation in any particular tissue or organ was not observed. In every tissue and organ of 24h after final repeated administration, each radioactive concentration was found to be higher than the corresponding value at 24h after a single oral administration.3) Transference of radioactivity to milk occurred, and the peak of concentration in milk was observed at 6h after oral administration; however, its ratio to the concentration in dam blood increased with time until 24h.4) Transference of radioactivity in bile began just after oral administration, but the amount was small.5) About 21% of the dose was excreted in expiration by 24h after single oral administration.6) Percutaneous absorption took place in animals with abraded skin much more than in animals with normal skin.7) In the dialysis experiment, 14C-diethylene glycol did not combine with normal plasma, but binding of radioactivity to plasma was noted in plasma 1h and 24h after oral administration.8) About 93% of radioactive urinary metabolites were identified as intact dethylene glycol, and the remaining 7% as oxalic acid. All radioactive expiration was the form of carbon dioxide.