BAT26 marker as detector of microsatellite instability in breast tumors

Abstract

Microsatellite are genetic loci that contain sequential repeats of consise motif of 1 to 6 or more nucleotide bases. The spontaneous decrease or increase of nucleotides from repetitive deoxyribonucleic acid segments is named microsatellite instability. The aim of this study was to detect MSI in breast neoplasms (ductal carcinoma) and benign breast tumor (fibroadenoma) by using BAT26 marker. Twenty-one benign tumor cases and twenty-three malignant tumor cases where included in this study and the formalin fixed paraffin embedded samples were collected from those cases. Ages of patients group ranged from 16 to 72 year with mean (43±2.1 years). The DNA was extracted from 25mg of FFPE samples from each tumor and normal tissue from all patients. BAT6 locus was amplified by using conventional PCR technique and the high-resolution agarose (HR) was used to detect MSI at BAT26 locus. The findings showed that the MSI at BAT26 locus in malignant group was (13%), while in benign cases was (9.5%). No significant (P> 0.05) correlation was found between MSI and (age, grade and hormonal receptor status) in malignant cases. In addition, there’s no significant correlation with age in benign cases. As a conclusion, MSI at BAT26 locus can be detected in both benign and malignant breast tumor cases.