Abstract
Negative or weak Feulgen reactions have been obtained in many plant organisms. The three procedures for staining with basic fuchsin presented in this paper were developed in consequence of failure to obtain adequate Feulgen reactions in cytologic studies on certain fungi pathogenic for man, and because of the difficulty encountered in obtaining iron alum hematoxylin preparations which do not have a murky aspect. Clear and beautiful preparations can be obtained by the following technics, which appear to hold tremendous promise for studies of nuclear phenomena in the fungi. Ohlmacher (1895) was the first and, we believe, the only author to use formaldehyde as a mordant for basic fuchsin staining. He applied his methods to bacteria and tissues. The studies of DeLamater and Ulrich extend, but only partially confirm, the initial observations of Ohlmacher.
Published Version
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