BAMBI inhibits inflammation through the activation of autophagy in experimental spinal cord injury.

Abstract

Autophagy plays an important role in the progression of spinal cord injury(SCI). In this study, we aimed to examine the effects and potential mechanisms of action of BMP and activin membrane-bound inhibitor(BAMBI) in the progression of SCI. A rat model of SCI was established and the rats were injected with pLentiH1-BAMBIshRNA and pAd-BAMBI in the gray and white matter of the spinal cord atT8. After 14days, motor function evaluation was measured according to the Basso Beattie Bresnahan(BBB) method and the number of motor neuron cell accounts in the anterior horns was measured by Nissl staining. The protein expression of levels light chain3B(LC3B), Beclin1, Bim and p62 were measured by western blot analysis. The concentrations of interleukin(IL)-1β, IL-6 and IL-10 were measured by ELISA. The results revealed that BAMBI expression was significantly decreased in the rats with SCI. The BBB scores and the number of motor neuron cell accounts in the anterior horns were significantly decreased in the pLentiH1-BAMBIshRNA injection group, and were increased in the pAd-BAMBI injection group, suggesting a neuroprotective effect of BAMBI in SCI. Moreover, the increased expression levels of LC3B and Beclin1, and the decreased expression of Bim and p62 indicated that autophagy was significantly induced in the pAd-BAMBI injection group. Moreover, the overexpression of BAMBI also decreased the expression of transforming growth factor-β(TGF-β) and mammalian target of rapamycin(mTOR), and decreased the concentrations of IL-1β, IL-6 and IL-10. These results indicate that BAMBI plays a neuroprotective role by decreasing inflammation and activating autophagy in rats with SCI.