Abstract
BackgroundBartonellosis due to Bartonella bacilliformis is a highly lethal endemic and sometimes epidemic infectious disease in South America, and a serious public health concern in Perú. There is limited information on the immunologic response to B. bacilliformis infection. The objective of this research was to produce experimental infection of BALB/c mice to B. bacilliformis inoculation.FindingsBALB/c mice were inoculated with 1.5, 3.0 or 4.5 × 108 live B. bacilliformis using different routes: intraperitoneal, intradermal, intranasal, and subcutaneous. Cultures of spleen, liver, and lymph nodes from one to 145 days yielded no cultivable organisms. No organs showed lesions at any time. Previously inoculated mice showed no changes in the reinoculation site.ConclusionParenteral inoculation of live B. bacilliformis via different infection routes produced no macroscopic or microscopic organ lesions in BALB/c mice. It was not possible to isolate B. bacilliformis using Columbia blood agar from 1 to 15 days after inoculation.
Highlights
Bartonellosis due to Bartonella bacilliformis is a highly lethal endemic and sometimes epidemic infectious disease in South America, and a serious public health concern in Perú
Bacteria were harvested in a laminar flow hood, scraping colonies off the agar surface into brain heart infusion (BHI) broth media
Factors involved in host specificity and ligand-receptor affinity can be involved in failure to reproduce infection by many Bartonella species in BALB/c mice [17]
Summary
Bartonellosis due to Bartonella bacilliformis is a highly lethal endemic and sometimes epidemic infectious disease in South America, and a serious public health concern in Perú. There is limited information on the immunologic response to B. bacilliformis infection. Carrion's disease is an infectious disease; endemic in some regions of Peru, Colombia and Ecuador [1]. The incidence of infection has been estimated at 12.7/100 person-years [2]. There is limited information on the immunologic response to Bartonella infection but it is widely known that antibodies are detectable in convalescent patients with long-term protective immunity [10,11,12]. The presence of chronic asymptomatic carriers in endemic areas and the appearance of the chronic phase contribute to the (page number not for citation purposes)
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