Abstract
In this study, the effects of Baicalin on the hyperglycemia-induced cardiovascular malformation during embryo development were investigated. Using early chick embryos, an optimal concentration of Baicalin (6 μM) was identified which could prevent hyperglycemia-induced cardiovascular malformation of embryos. Hyperglycemia-enhanced cell apoptosis was reduced in embryos and HUVECs in the presence of Baicalin. Hyperglycemia-induced excessive ROS production was inhibited when Baicalin was administered. Analyses of SOD, GSH-Px, MQAE and GABAA suggested Baicalin plays an antioxidant role in chick embryos possibly through suppression of outwardly rectifying Cl(−) in the high-glucose microenvironment. In addition, hyperglycemia-enhanced autophagy fell in the presence of Baicalin, through affecting the ubiquitin of p62 and accelerating autophagy flux. Both Baicalin and Vitamin C could decrease apoptosis, but CQ did not, suggesting autophagy to be a protective function on the cell survival. In mice, Baicalin reduced the elevated blood glucose level caused by streptozotocin (STZ). Taken together, these data suggest that hyperglycemia-induced embryonic cardiovascular malformation can be attenuated by Baicalin administration through suppressing the excessive production of ROS and autophagy. Baicalin could be a potential candidate drug for women suffering from gestational diabetes mellitus.
Highlights
Introduction Roots ofScutellaria baicalensis are used in Chinese medicine and it reputedly calms fetuses in pregnant women[1,2]
Dysplasia of the cardiovascular system during cardiogenesis and angiogenesis leads to constantly impaired fetal development or death
The developing chick embryos treated with sample saline or various concentrations (3, 6, 12 and 24 μM) of Baicalin were photographed at incubation times of 0, 26, 39 and 48 h (Fig. 1b)
Summary
Introduction Roots ofScutellaria baicalensis are used in Chinese medicine and it reputedly calms fetuses in pregnant women[1,2]. Baicalin is principally used in Asian countries as a herbal supplement because of its wide variety of health benefits, including anti-neuroinflammatory[3], anticancer[4] and anti-anxiety effects[5]. It can improve lung function[6] and fertility[7].The most common mechanism of Baicalin being against pathogenesis is its protective effect by reducing the production of oxidative stress[8]. This study focuses on the potential beneficial effects of Baicalin on the early development of heart tube and vasculature, in order to explore whether or not Baicalin could be a potential candidate drug for pregnant women
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
