Badanie poziomu emisji fluorescencji produktów reakcji wybranych aminokwasów z DFO, 1,2-indanedione oraz 1,2-indanedione z chlorkiem cynku

Abstract

The study shows the results of fluorescence emission intensity measurements of the reaction products of selected amino acids with DFO, 1,2-IND and 1,2-IND - zinc chloride on absorptive surfaces. Conducted research addressed the following variables: type of developing reagent, type of surface, type of amino acid and sample storage time. It was confirmed that the above factors affected the fluorescence intensity of developed fingerprints. Furthermore, the studies proved that fluorescence intensity of reaction products between amino acids and 1,2- IND stored for 1 or 7 days increased for the majority of samples upon addition of zinc chloride to the developing reagent. For samples stored for 4 months the highest fluorescence emission intensity was observed for DFO. No significant differences were found in fluorescence spectral characteristics of tested compounds, depending on the type of surface.