Profile of Kerandang Fish (Channa pleurophthalmus Blkr) Proteins from Central Kalimantan

Abstract

Kerandang fish (Channa pleurophthalmus Blkr) as one of freshwater fish from the Channidae family which is very potential and abundant in Central Kalimantan. The aim of this research was to determine and obtain a picture of the protein profile Kerandang fish. The benefit of this research is that Kerandang fish can be utilized maximally as one of the nutritious foods to demand the nutritional needs of the people in Central Kalimantan. This research was conducted exploratively qualitative with the parameters of the proximate test, amino acids using HPLC and determination of protein molecular weight using SDS-PAGE. The results obtained for the proximate test were 76.48% water content, 16.35% protein content, 0.35% fat content and 6.82% carbohydrate content. The results for amino acids assay obtained 15 types of amino acids, 8 types of essential amino acids with the highest type of amino acid, Leucine 4.69% and 7 types of non-essential amino acids with the highest type of amino acid, glutamic acid 9.11%, and total acid amino 53.32%. The result of analysis of protein profile of Kerandang fish meat based on its molecular weight using SDS-PAGE there are 27 protein bands in the range of molecular weights 10.202 - 134.432 kDa.