Bactericidal and Sporicidal Activities against Pathogenic Bacteria of Direct Flow Electrolyzed Water

Kataoka Y,Hatakeyama H,Sawada T,Ochi H,Miyakawa Y

doi:10.4172/2572-4134.1000105

Abstract

The purpose of this investigation was to examine the bactericidal activity of long lifetime ozone water (LLO water) against pathogenic bacteria of medical, veterinary, and public health interest. An electrolytic system using Pt mesh electrodes combined with a conventional polymer electrolyte, the Nafion 117 membrane, produced LLO water from tap water with high efficiency. LLO water killed 7.144 ± 0.0507 log10 CFU of Escherichia coli O157:H7, 6.979 ± 0.045 log10 CFU of methicillin-resistant Staphylococcus aureus, 6.503 ± 0.3681 log 10 CFU of Pseudomonas aeruginosa and 6.579 ± 0.123 log 10 CFU of Legionella pneumophila within one minute. In contrast, endospores of Bacillus anthracis were killed within 20 min. Results of observation of the bactericidal activity of LLO by using an electron microscope, it was revealed that LLO water was shown the bactericidal action by swelling and destroying on the cell wall of the bacterial cells. Our studies demonstrated that LLO water has the potential to be widely applicable in food hygiene, medicine and veterinary medicine as a sterilizing agent. This is the first study to demonstrate the bactericidal activity and characteristics of LLO water.

Highlights

Functional water produced by electric systems, such as electrolyzed water, has been widely used as a safe and low cost bactericidal water compared with disinfectants
S. aureus NVLU183 was cultured in trypticase soy broth at 37°C for 18 hrs, and 200 l of each bacterial culture was mixed with 20 ml of Long-lifetime ozone (LLO) water or 20 ml of distilled water
LLO water, which was produced from tap water, was shown to work as well as bactericidal disinfectants against various pathogenic bacteria found in medical and public health settings

Summary

Introduction

Functional water produced by electric systems, such as electrolyzed water, has been widely used as a safe and low cost bactericidal water compared with disinfectants. We have developed a new electrolytic water generated by the system to direct electrolysis of tap water [2] This electrolyzed water, named long-lifetime ozone water (LLO water), has a neutral pH and contains high concentrations of ozone and hydroxyl radicals, without NaClO [2]. The LLO water is long-lifetime ozone water; it is possible to inactivate many pathogenic agents of medicine, veterinary medicine and public health concern. Major etiological agents of nosocomial infections include methicillin-resistant S. aureus (MRSA), multidrugresistant P. aeruginosa (MDRP) and L. pneumophila [7,8,9] These organisms are problematic because of their ubiquitous nature, ability to survive in the hospital environment, and innate resistance to many antibiotics and antiseptics. The characteristics of LLO water were determined by carrying out dilutions and limited values

Material and Methods

Results and Discussion

Discussion