Bactericidal activity of zinc sulphate bio-nanoparticles against enterobacteriaceae pathogens

Abstract

In order to find out the solution to prevent the growth of antibiotic resistant bacteria as well as the clinical isolates of enterobacteriaceae family pathogens, zinc sulphate bionanoparticles were synthesized from one of the airborne allergenic fungus viz., Aspergillus niger isolated from indoors of the MGR College, Hosur, TN, India during 2010. Antibacterial assay of such zinc nanoparticles were analyzed against enterobacteriaceae pathogens i.e., Escherichia coli, Klebsiella pneumonia, Shigella dysenteriae, Salmonella typhii and Proteus mirabilis through minimum inhibitory concentration and well diffusion methods. For the present study, clinical isolates of above said bacteria were obtained from Government General Hospital, Hosur, TN and later confirmed by various biochemical observations. Inhibition of pathogenic organisms was found in all the tubes from 1∶2 to 1∶512 dilutions. Zinc nanoparticles showed higher inhibition effect at different concentrations. Shigella dysenteriae was found as the susceptible clinical isolate to the zinc nanoparticles at all the concentrations 25 µl–100 µl. Likewise P. mirabilis (16mm) was more inhibited at 25µl concentration, S. typhii (21mm) at 50µl, E. coli (20mm) at 75 µl and P. mirabilis (25mm) as well as S. typhii (24mm) were found sensitive to the zinc nanoparticles at 100µl concentration. Other bacterial isolates were more or less sensitive to the inhibition effect.