Antibacterial Effect of Dill Seed Oil Anethum graveolens Anethum graveolens

Abstract

Background: study the inhibiter activity of Dill seed oil A. graveolens L. on growth of some pathogenic bacteria. Objective: assay the inhibition effectiveness of Dill seed oil on some pathogenic bacteria which causes different infections. Methodology: an experiment was run to study the antibacterial activities of Dill seed oil on six isolates of bacteria: E. coli, K. pneumonia, P. mirabilis, P. aeruginosa, S. aureus, , and E. faecalis which isolated of patients suffer of different infections of both genders their age ranged (15-50) year, used agar well diffusion method. The isolates selected of Baghdad Teaching Hospital-Teaching Laboratories in December 2014 to the February 2015. Data was analyzed by using the Lycart scale analysis, and assays the Minimum Inhibitory Concentration (MIC). Results: Dill seed oil significantly reduced the growth of test bacteria: E. faecalis (15-25mm), P. mirabilis (20-25mm), P. aeruginosa (17-27mm), K. pneumonia (15-35mm), E. coli (20-52mm), and the widest inhibition zone diameters was showed on S. aureus (70-72mm). Conclusion: Dill seed oil has clear inhibition effect on the growth of Gram-positive and Gram-negative pathogenic bacteria. Recommendation: done further studies on dill seed oil for development of novel drugs of A. graveolens to treat human diseases as a result of its inhibition effectiveness on growth of bacteria and decreasing the use of antibiotics because the problem of resistant of bacteria and side effects, and it is necessary to determine the toxicity of the active constituents and pharmacological -kinetic properties.