Abstract
The phenomenal rise in the demand of biosensors accelerated their rapid development and immersive applications in the myriads of fields. The essential requirement of developing efficient bio-sensing platform is to find stable well organized interfacial architecture that can serve as an excellent matrix for binding and recognizing biomolecules. In this context, the enormous potential has been envisaged in surface layer proteins that represented themselves as most primitive and simplest self-assembled system with repetitive physicochemical properties for the molecular functionalization of surfaces and various interfaces. The prominence of S-layer proteins has been broadened by integrating genetic engineering approaches for the fine tuning of functional groups and protein domains in geometrically well-defined manner. The efficient and stable binding of various nanomaterials with S-layers in regular arrays has led to paradigmatic shift in their nano-biotechnological sensing applications. More recently, functional S-layer supported lipid membranes have been generated through covalent binding of lipid molecules either with native or recombinant S-layer proteins at nano-scale dimensions serving as “proof of concept” for the development of bio-sensing platform. Thus, in the light of benefits conferred by surface layer proteins for the development of highly efficient biosensors, an exciting path has been opened for broadening their translational applications in drug delivery, disease diagnosis, vaccines development, lab-on-chip devices etc. Therefore, this review intends to describe about the importance of surface layer proteins in the development of biosensors.
Highlights
The past decade has seen expanding repertoire of bio-sensing devices that have been explicitly utilized in the field of biomedicine, diagnostics, food processing and safety, forensic sciences, security and environmental monitoring [1] [2] [3]
In the light of benefits conferred by surface layer proteins for the development of highly efficient biosensors, an exciting path has been opened for broadening their translational applications in drug delivery, disease diagnosis, vaccines development, lab-on-chip devices etc
This review intends to describe about the importance of surface layer proteins in the development of biosensors
Summary
The past decade has seen expanding repertoire of bio-sensing devices that have been explicitly utilized in the field of biomedicine, diagnostics, food processing and safety, forensic sciences, security and environmental monitoring [1] [2] [3]. The advent of metabolic engineering and synthetic biology has paved the way for extensive development of in vivo biosensors that are based on biomolecular architecture comprising either proteins or nucleic acids [4]. These biosensors can be developed by the engineering of existing components for novel functions through rational design approaches and directed evolution, or directly obtained from genome mining. The current review describes the recent advancements, current bottlenecks and future prospects of biosensors based on surface layer proteins
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
