Abstract
Rice is currently the most important food crop in the world and we are only just beginning to study the bacterial associated microbiome. It is of importance to perform screenings of the core rice microbiota and also to develop new plant-microbe models and simplified communities for increasing our understanding about the formation and function of its microbiome. In order to begin to address this aspect, we have performed a 16S rDNA taxonomic bacterial profiling of the rhizosphere and endorhizosphere of two high-yield rice cultivars—Pionero 2010 FL and DANAC SD20A—extensively grown in Venezuela in 2014. Fifteen putative bacterial endophytes were then isolated from surface-sterilized roots and further studied in vitro and in planta. We have then performed inoculation of rice seedlings with a simplified community composed by 10 of the isolates and we have tracked them in the course of 30 days in greenhouse cultivation. The results obtained suggest that a set was able to significantly colonize together the rice endorhizospheres, indicating possible cooperation and the ability to form a stable multispecies community. This approach can be useful in the development of microbial solutions for a more sustainable rice production.
Highlights
Rice is the staple food for more than a half of the world population and its production is dependent on chemical fertilizers and pesticides [1] which are in part responsible for global warming and groundwater pollution [2]
Rhizosphere bacteria, which live in the soil that is in intimate contact with the roots, are able to perform beneficial functions and these are known as plant-growth promoting rhizobacteria (PGPR) [5]
In order to obtain a picture of the taxonomic diversity of the two Venezuelan rice cultivars, the total rhizospheric and endorhizospheric bacterial community was assessed
Summary
Rice is the staple food for more than a half of the world population and its production is dependent on chemical fertilizers and pesticides [1] which are in part responsible for global warming and groundwater pollution [2]. To meet the world’s demand for rice it is imperative to find environmentally sound ways that supplement the need for fertilizers [3]. Some rhizospheric bacteria are capable of penetrating the surface of the roots and colonizing the internal tissues of the root (a compartment known as the endorhizosphere [6]) and these are called endophytes. These bacterial endophytes overcome plant defences and establish themselves as permanent inhabitants of internal tissues without causing harm to the host plant [7]. It is believed that bacterial endophytes interact closely with the host having less competition for nutrients and living in a more protected environment [8]
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