Abstract
Transcription levels of amoA mRNA and 16S rRNA during an aeration phase in SBR (sequencing batch rector) were analysed using reverse transcription PCR (RT-PCR) and a relationship between amoA mRNA expression in activated sludge and changes in nitrogen compounds concentrations was examined. Expression of amoA gene reached a detectable level two hours after a beginning of the aeration phase and did not disappear until its end. Lack of detectable amoA expression at the beginning of the aeration phase was in agreement with nitrite concentration decrease. Gradual increase in amoA transcripts level observed during next hours indicated a rise in ammonia-oxidising bacteria activity, a detectable change in nitrite concentration was observed 2 h after the RT-PCR signal was provided for the first time. Changes in 16S rRNA transcription level indicated that metabolic activity of the activated sludge bacterial community increased gradually during the aeration phase.
Published Version
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