Abstract
The current research tried to explore the effect of Qiweibaizhu powder (QWBZP) on the bacterial diversity and community structure of the intestinal mucosa of dysbiosis diarrhea mice and provide a scientific basis for the efficacy of QWBZP on antibiotic-induced diarrhea. A dysbiosis diarrhea mouse model was constructed with broad-spectrum antibiotics through a mixture of cephradine capsules and gentamicin sulfate (23.33 mL·kg−1·d−1). Intestinal mucosa was collected, and DNA was extracted from each group. The bacterial characteristics in intestinal mucosa were analyzed by MiSeq sequencing based on the 16S rRNA sequencing platform. There were no significant differences in alpha diversity indices among the three groups. The sample distributions in both the normal and QWBZP groups were relatively concentrated, and the distance among individuals was close. However, an opposite result was obtained in the model group. Furthermore, the composition and abundance of species were similar between the normal group and the QWBZP group at both the phylum and genus levels. After treatment with QWBZP, the abundance of Lactobacillus increased, and Proteobacteria decreased, and the Firmicutes/Bacteroidetes ratio decreased to a normal level. Our results indicate that QWBZP can help repair mucosal bacterial structure and recover mucosal microbiota. Specifically, QWBZP increased the abundance of Lactobacillus and Bacteroidales S24-7 group norank.
Highlights
The human intestine is inhabited by a large number of bacteria that adhere to the surface of the intestinal mucosa and participates in many physiological functions of the host, such as digestion, metabolism, immune regulation, energy conversion, and mucosal development and barrier maintenance [1,2,3,4]
We have studied the effect of Qiweibaizhu powder (QWBZP) on the bacterial lactase genes, but the functional lactase function gene cannot be detected in all bacteria, only lactase-producing bacteria; 16S rDNA can be detected in all bacteria
We aimed to investigate the effect of QWBZP on dysbiosis diarrhea from the diversity and community structure of microbiota in intestinal mucosa and provide more powerful evidence for the ability of Chinese medicine to alter the intestinal microbiota to improve intestinal mucosal barrier function
Summary
The human intestine is inhabited by a large number of bacteria that adhere to the surface of the intestinal mucosa and participates in many physiological functions of the host, such as digestion, metabolism, immune regulation, energy conversion, and mucosal development and barrier maintenance [1,2,3,4]. Intestinal bacteria live in a relatively stable dynamic balanced environment Once this balance is destroyed, the type, quantity, proportion, and location of intestinal bacteria will be disordered, resulting in dysbiosis, which will lead to pathological changes in the host [7]. The intestinal mucosa is the largest contact surface between the body environment and the intestinal cavity It consists of mucosal epithelial cells, tight intercellular links, and bacterial membranes. Chinese medicine has reduced the intestinal mucosal permeability, promoted intestinal peristalsis, promoted the growth of beneficial bacteria, inhibited the growth of spoilage bacteria, stimulated the secretion of intestinal mucosa-related cells, and enhanced intestinal immunity by regulating intestinal microbiota, which improved intestinal
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