Bacterial Degradation of Synthetic Dye by Pseudomonas sp. Obtained from Dyeing Mill Effluent

doi:10.34104/ajpab.020.01920199

Abstract

Environmental pollution is one of the major concerns of today’s world. Due to rapid industrialization and urbanization, a large number of wastes are generated and discharged into the environment and causing major pollution problems. For obtaining reactive dye decolorizing bacterial isolates, effluent samples were collected from a dyeing mill. From bacterial pure culture, 10 isolates were selected for screening. Screening of these isolates for the capability to decolorize and degrade reactive dye was performed in a nutrient broth medium containing reactive dye. 6 isolates among these bacterial isolates showed dye decolorizing ability within 120 hours of incubation. These isolates were further identified based on cultural, morphological, and biochemical characteristics. These characteristics indicated that these six bacterial isolates were distributed to the bacterial genus of Bacillus (2 isolates), Pseudomonas (2 isolates), Aeromonas (1 isolate), and Alcaligenes (1 isolate). For the study of dye decolorization and degradation, Reactive Dark Blue dye used in jute and textile industries was chosen. Pseudomonas, a prominent dye decolorizing isolate during screening, was taken for the optimization of different physicochemical parameters. This bacterium decolorized and grew well up to 500 mgL-1 of Reactive Blue dye. Pseudomonas sp. showed noteworthy decolorization of approximately 84% at 200 mgL-1 of dye concentration after 96 h of incubation. The optimum temperature for dye degradation was at 37 °C. The maximum level of decolorization for Pseudomonas sp. was observed at pH 8.0. This isolate showed better decolorization extent under static conditions rather than shaking conditions. This result indicated that the dye had been utilized by this bacterial isolate. It can be concluded that Pseudomonas is a prospective candidate in the biodegradation of Reactive Blue dye and might be useful in bioprocess technology used for the bioremediation of dyeing mill effluents.

Highlights

Effluent from dyeing industries is one of the main sources of aquatic environmental pollution among many pollutants
It can be concluded that Pseudomonas is a prospective candidate in the biodegradation of Reactive Blue dye and might be useful in bioprocess technology used for the bioremediation of dyeing mill effluents
Isolation, screening and identification of reactive dye decolorizing bacterial isolates – Heterotrophic bacteria were isolated from dyeing mill effluent

Summary

INTRODUCTION

Effluent from dyeing industries is one of the main sources of aquatic environmental pollution among many pollutants. Inappropriate release of effluents containing reactive dyes has detrimental effects on the aquatic environment for metals, aromatics, chlorides, etc. Bangladesh is second in garment-manufacturing and this is the largest sector for earnings foreign currency and employment generation (Farhana et al, 2015; Shuchismita and Ashraful, 2015) These industries use huge amount of dyes and discharge wastewater without treatment (Chindah et al, 2004) and the parameters for physicochemical property are found much higher than the recommended value of Department of Environment (Shuchismita and Ashraful, 2015). For the remediation of reactive dye generated toxicity, a bioprocess - that is effective and sustainable is needed and obtain-ning reactive dye-degrading bacteria from effluent is significant. Isolation of bacteria from effluent of untreated dyeing industry was performed in the present study. Reactive Blue dye applied in dyeing purpose in jute and textile industries was used for optimizing various physicochemical parameters for decolorization

MATERIALS AND METHODS

RESULTS AND DISCUSSION

CONCLUSION

REFERENECES