Abstract
How does a bacterial cell without a nucleus, without mitosis, and without extensive sister cohesion, organize, replicate, repair and segregate its chromosomes? We use live-cell super-resolution PALM and 3-D SIM imaging, alongside classical biochemistry and molecular genetics, and in vitro single-molecule biophysical techniques to address these questions. The presentation will focus on the sequential and coordinated action of Topoisomerase IV and the SMC complex, MukBEF, in E. coli chromosome segregation.ReferencesScience (2012) 338, 528-531PNAS (2013) 110, 8063-8068Nature (2014) 506, 249-253
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