Abstract

The rapid degradation of alginate beads limits the lifespan of immobilized cells. In this study, bacterial cellulose (BC) incorporated in alginate was used to improve the mechanical properties, swelling ratio, and recycling time of the immobilized photosynthetic bacterium Rhodopseudomonas faecalis PA2 for the removal of cooking oil residues. Beads reinforced with 25 and 50% BC showed a higher Young's modulus and compressive strength and a lower swelling ratio than the control treatment (0% BC). The incorporation of 50% BC increased biomass production and oil removal. Field-emission scanning electron microscopy revealed several bacteria-infested internal pores in the reinforced beads, indicating bacterial growth in the presence of BC. Bacterial viability was verified by BC immersion in the bacterial culture broth and by injecting bacteria into the BC matrix. Without BC reinforcement, beads collapsed after reuse in two batches, whereas reinforced beads could be reused for five batches, resulting in an oil removal rate of up to 76.3 %. Our results show that BC can be used as an alginate reinforcing material to improve bead stability and prolong the effective recycling period of immobilized bacteria without negatively affecting bacterial growth or waste oil removal.

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