IN VITRO STUDY OF THE EFFECTS OF BACTERIAL CELLULOSE, GLUCOSE, AND TESTOSTERONE ON THE OSTEO‐1 CELLS MULTIPLICATION

Abstract

The objective of this study was to analyze the cell multiplication during 15 days of culture, in the absence or presence of bacterial cellulose (BC), in hyperglycemic and hyperandrogenic mediums. OSTEO‐1 cells were cultured with 1.5 mg / L and 4.5 mg / L glucose (mimicking normal glycemia and hyperglycemia), in the absence and presence of two concentrations of testosterone (T) (10−9 M and 10−3 M, mimicking normal androgenemia and hyperandrogenemia), in the absence and presence of BC. Cells were cultured at an initial density (day 0) of 2000 cells per 200 μL of osteogenic medium (DMEM + FBS + ascorbic acid + dexamethasone + b‐glycerophosphate), in 48‐well culture plates, for 5, 10, and 15 days, in triplicates, with exchanges of the medium every 72 h. Cell counting was performed with a Bio‐Rad TC20™ cell counter. The data were analyzed using ANOVA and Fisher's test for multiple comparisons. Culture time effects: The highest cell counts were observed at 15 days, except for the BC group in the presence of T at 10−9 M in hyperglycemic medium, which presented a higher cell count at 10 days of culture. The lowest cell counts were observed at 5 days of culture. Hyperglycemic medium effects: At 5 days of culture, the only treatment that did not present a higher cell count was BC control. At 10 days, all the treatments without BC, as well as the control with BC, showed lower cell counts, while both treatments with BC and T presented higher cell counts. At 15 days, lower cell counts were observed for the control group without BC and for the BC + T at 10−9 M and BC + T at 10−3 M groups. The other treatments presented higher cell counts in the presence of the hyperglycemic medium. BC and T effects. At 5 days of culture, the highest cell counts were observed in the presence of BC and T, while the lowest counts were in the absence of BC. At 10 days, the highest cell counts were observed for the BC + T groups, while the lowest counts were observed for the groups with BC, in the absence of T. At 15 days of culture, the highest cell counts were observed for the group with BC, in the absence of T, and for the group with BC + T at 10−3 M, while the lowest cell counts were observed for the groups without both CB and T. The results indicated that the cells presented higher multiplication activity at 15 days of culture in the presence of BC. In addition, the cell multiplication activity was stimulated by the hyperglycemic medium and by the T in a time‐dependent and dose‐dependent manner. Hence, BC can be used in OSTEO‐1 cultures for studies of hormonal and metabolic effects related to cell multiplication.Support or Funding InformationFAPESP (Grant 2018/14840‐4)This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.