Background formation of lower levels of reactive oxygen species by neutrophils after hypomagnetic field exposure is not accompanied by the impairment of chemiluminescence response of neutrophils to respiratory-burst stimuli

Abstract

This study shows that the background formation of lower levels of reactive oxygen species in mouse peritoneal neutrophils after short-term (40 minutes) exposure to hypomagnetic fields with ~10 nT residual field at physiological temperatures, which has been detected by use of lucigenin-dependent chemiluminescence, is not accompanied by the impairment of chemiluminescence response of neutrophils to respiratory-burst stimuli: the formylated tripeptide N-formyl-Met-Leu-Phe (fMLF) and phorbol ester phorbol-12-myristate-13-ace-tate (PMA). These results were obtained with lucigenin or luminol-enhanced activated chemiluminescence and various combinations of reactive oxygen species production stimuli (phorbol-12-myristate-13-acetate and/or N-formyl-Met-Leu-Phe). Based on the results of the present work as well as on those of previous studies, this study reveal that the systems that control the respiratory burst in neutrophils can be excluded from a list of main targets and acceptors that respond to short-term deprivation of the magnetic field.