Abstract
By using GC-MS and HPLC-MS methods, alkaloids have been investigated in the Datura stramonium (thorn apple) from the flora of Azerbaijan. Totally 40 nortropane, tropane and pyrrolidine derivatives have been identified in the plant samples. Atropines have been quantified in the aerial parts of plant by using HPLC-DAD method in comparison with analytical standard of substance. Atropine was 4.353 mg/g in the dried plant sample. Spermidine and tyramine derived natural polyamines have been detected in the aerial parts of plant by performing HPLC-MS method. Sterols have been tested in the seed oil of plant in comparison with analytical standards of cholesterol, kaempesterol, stigmasterol and β-sitosterol by using GC-FID method. ICP-MS have been performed for qualitative and quantitative determination of 6 macroelements and 13 microelements in the roots, stems, leaves, flowers, fruits and seeds of plant. Along with heavy metals, several elements, which have macro- and micronutrient importance, have been determined in the samples.
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