Abstract
Axin interactor, dorsalization-associated (AIDA) is a protein that interacts with Axin and involved in the regulation of embryonic development, intestinal fat absorption, and adaptive thermogenesis in brown adipose tissue. However, the roles of AIDA in feeding behavior and hepatic glycolipid metabolism in the fish remain uncovered. In this study, the AIDA gene of Schizothorax prenanti (S. prenanti) was cloned and the recombination AIDA protein of S. prenanti was obtained using a prokaryotic expression system. The highest expression level of the AIDA gene was detected in the hypothalamus, followed by the cerebral cortex, liver, muscle, and heart. Fasting induced the expression level of AIDA gene in the liver, cerebral cortex, and hypothalamus, but refeeding attenuated the AIDA expression. Furthermore, the recombinant AIDA protein (200 ng/g BW) i.c.v. injection increased the food intake of S. prenanti by increasing the expression levels of orexigenic factors (AgRP and Ghrelin) and decreasing the expression of anorexigenic factors (Cart, CCK, and Leptin). In addition, the i.p. injection of recombinant AIDA protein significantly decreased the glucose, TC, and TG contents in plasma and reduced the glycogen and TG contents in liver. These results demonstrate that AIDA protein increases appetite and regulates hepatic glycolipid metabolic processes in S. prenanti.
Published Version
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