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Abstract
In order to assess the proliferative behaviour of the ameloblastoma epithelium nine cases of this neoplasm were retrieved from the files of the Oral Pathology Service laboratory of Rio Grande do Sul Federal University School of Dentistry. Two proliferation marker techniques were employed: the AgNor - argyrophilic nucleolar organizer regions staining and the proliferation cell nuclear antigen (PCNA) immunohistochemical assessment technique. Each case was submitted to a quantitative and comparative statistical analysis of the results obtained for the two techniques. The conclusion arrived at shows ameloblastoma presenting benign behavior, an average AgNOR index of 1.612, and a PCNA proteindependent proliferative activity with average immunoreactivity of 51.61%. The histological patterns analyses of the neoplastic epithelial component demonstrates that cells resembling pre-ameloblasts have obtained figures statistically higher to those obtained for cells resembling the stellate reticulum of the enamel organ, in both techniques employed. Therefore, it might be inferred that cells resembling pre-ameloblasts hold the proliferative potential of ameloblastomas and are probably responsible for the growth characteristics and infiltrative ability of this tumour.
Highlights
argyrophilic nucleolar organizer regions (AgNORs) index of L6I2, and a proliferating cell nuclear antigen (PCNA) proteindependent proÌiferative activity with average immunoreactiúty of 5 I.ól o/o
KOBAYASHI, I et al The proliferative activity in oral epithelial dysplasia analysed by proliferating cell nuclear antigen immunostaining and argyrophilic nucleolar organiser region staining
Figura 2 - Fotomicrografia (400X) de um campo microsípico de ameloblastoma (área plexiforme) onde podemos observar a expressão imunohistoquímica da proteína proliferating cell nuclear antigen (PCNA) nos núcleos das céÌuÌas assemelhadas aos pré-ameloblastos (+) e células assemelhadas às do reúculo esüelado do órgão do esmalte (+)
Summary
Onofre Francisco de Quadros** João Jorge Diniz Barbachan** Manoel Sant'Ana Filho* *. MEDEIROS, FRXITAS e SOUZA17, ROSA, JAEGER e JAEGER30 e Do CARMO e SILVAó por sua vez interpretaram os baixos valores médios de AgNORs. obtidos pelos ameloblastomas como um resultado da ausência de uma atividade proÌiferativa celular passível de ser conelacionada ao comportamento clínico agressivo característico do ameloblastoma, Porém, consideramos essa capacidade proliferativa das células dos ameloblastomas bastante signficativa baseados em nossos resuÌtados com o marcador. Quando ana.Iisamos separadamente os dois tipos celulares do ameloblastoma, quanto à expressão da proteína PCNA, notamos que as células âssemelhadas aos pré-ameloblastos possuem uma atiüdade proliferativa sigrrifi cantemente maior (37,880/o) que as células assemeÌhadas as do reúculo estrelado (13,73o/o), indicando que as primeiras são possivelmente as detentoras da capacidade proMerativa deste tumor. Já nos trabalhos para identficação da expressão da proteína PCNA, todos os autores procuraxam contar ambos os tipos celulares; mas apenas TAKAHASHI et a1.33 quantificaram e analisÍìram-nas separadamente, obtendo resultados simiÌares aos deste estudo
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