Abstract
Introduction: This experimental study gauged the value of argyrophilic nucleolar organizer region (AgNOR) staining as a possible technique for the estimation of cell kinetics in conventional histology sections, in benign and malignant breast lesions. Methods: With a silver staining technique and immunohistochemistry, we associated the numbers of AgNORs and Ki67 scores in 30 breast carcinomas and 10 benign breast lesions. Results: The mean values of Ag NORs silver stain dots count for normal, benign, grade II and III were 1.28±0.17, 2.83±0.68, 5.23±0.87 and 7.32±0.92, respectively. There was a statistically significant difference (p≤0.001) was noticed between the all individual groups, among the normal and breast lesion as well as among the GII and GIII. Immunohistochemical Results of Ki-67 protein exhibited homogenous golden-brown color in control case and a positive brown granules or diffuse dark brown color in the nuclei of both benign and malignant cases under the 400X magnify examined under the light microscope. Discussion: AgNOR counts performed on routine formalin-fixed paraffin sections could provide substantial kinetic evidence. Additionally, the difference in AgNOR counts between benign and malignant tumors is such that they may be of diagnostic worth.
Highlights
This experimental study gauged the value of argyrophilic nucleolar organizer region (AgNOR) staining as a possible technique for the estimation of cell kinetics in conventional histology sections, in benign and malignant breast lesions
We evaluated the correlation of Ki-67 and argyrophilic nucleolar organizer regions (AgNORs) staining expression as proliferation markers in both benign and malignant breast lesions with hormonal receptor status and other clinicopathological parameters
The current outcomes of malignant group showed that 90% of the breast cancer cases were invasive ductal carcinoma (IDC), most of which were allocated to the age range of (>35-55) years and tumor size T2 (>2-5) cm was present (72.2%, 13/18) of GII and (6/12-50.0%) of GIII and lymph node involvement present in (91.6%) of GIII and (8.4%) of GII of malignant group
Summary
This experimental study gauged the value of argyrophilic nucleolar organizer region (AgNOR) staining as a possible technique for the estimation of cell kinetics in conventional histology sections, in benign and malignant breast lesions. Its core drawbacks are that frozen sections or fresh cells are needed, it does not permit identification of the proportion of cells occupying each phase of the cell cycle, other than the GO-phase, and it does not measure the length of the cell cycle Because it is a relatively simple technique, Ki67 evaluation is the most popular method used to measure human tumor kinetics. We evaluated the correlation of Ki-67 and argyrophilic nucleolar organizer regions (AgNORs) staining expression as proliferation markers in both benign and malignant breast lesions with hormonal receptor status and other clinicopathological parameters
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