Abstract
Auxin Control of Root Organogenesis from Callus in Tissue Culture.
Highlights
Using leaf explants of Arabidopsis (Arabidopsis thaliana), adventitious roots could usually be regenerated by two ways: adventitious roots could be formed directly from detached leaf explants when cultured on B5 medium without added hormones (Chen et al, 2014; Liu et al, 2014), hereafter called direct De novo root regeneration (DNRR) (Figure 1A); or adventitious roots could be formed from callus in tissue culture, hereafter called indirect DNRR
In indirect DNRR, leaf explants are first cultured on callus-inducing medium (CIM) with high auxin levels to induce callus formation, and the callus is transferred to root-inducing medium (RIM) with low auxin levels or even on B5 medium without auxin supplement to allow root formation (Figure 1B)
While the cell fate transition during direct DNRR from Arabidopsis leaf explants has been carefully studied in Arabidopsis (Liu et al, 2014; Chen et al, 2016b,c; Hu and Xu, 2016; Sheng et al, 2017), in indirect DNRR the cell fate transition is still not clear
Summary
Reviewed by: Shuang Wu, Fujian Agriculture and Forestry University, China Akira Iwase, RIKEN, Japan
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